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Cultural and nutritional conditions for Lyophyllum decastes and its chemical composition in a apathetic medium were investigated. The optimal temperature and pH for the production of mycelium were 25℃ and pH 7.5. The mycelium yield was the highest on 15th day. Among the carbon sources, glucose and CMC were the best for the production of mycelium and their optimal concentrations were 3 and 6%, respectively. As an organic nitrogen, proteose peptone was the best and (NH₄)₂HPO₄ as an inorganic nitrogen was good. The optimal concentration of proteose peptone and (NH₄)₂HPO₄ were 2 and 0.2%, respectively. The optimal ratio of glucose to proteose peptone for production of mycelium was 10 : 1. Also, the optimal concentrations of K₂HPO₄ and MgSO₄ were 0.2 and 0.06%, respectively and that of CaCl₂ was 0.1%. Among the bioextracts, yeast extract was the most effective and its optimal concentration was 1.5%. In chemical components of the mycelium of Lyophyllum decastes, total sugar, crude protein and crude fat were 34.80, 28.35 and 2.50%, respectively. Its ash was 7.57% and crude fiber 11.99%.
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